Dr. Andrea Szuchman-Sapir

Vascular Signaling Research

Researcher, PI

Head of The Department of Nutrition and Natural Products

Head of Department

Research Group Leader

Ph.D.

Phone

972-4-6955029

andreas@migal.org.il

Endothelial dysfunction; EDHF; CYP-epoxygenase; vascular dilation; PON1; PUFA

Our research focuses on elucidation signal transduction mechanisms in the vessel wall, in particular in the endothelium, and on the generation of specific PUFA- derived metabolites that are potential endothelium-derived hyperpolarizing factors (EDHFs).

Current projects aimed to elucidate the role of cytochrome P450-derived specific lipid mediators in vascular homeostasis and dilation that affects blood pressure. In addition, the role of paraoxonase 1 in the endothelium signaling cascades that affect endothelium-dependent relaxation is been investigated.

Education
Ph.D. 2006, Technion Faculty of Medicine, Haifa, Israel.

M.Sc. 2000, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Academic and research positions

2017-present, Head of the Laboratory of vascular signaling research, Migal-Galilee Research Institute

2017-present, Head of the Institutional Review Board (IRB) at the Tel-Hai College, Israel

2016-present, Senior lecturer at the Tel-Hai College, Israel

2009-2016 Scientist at the Human Health & Nutrition Sciences laboratory, Migal-Galilee Research Institute

2007-2009: Post-Doctoral at the Vascular Biology Group, ANZAC Research Institute, Concord Hospital, in collaboration with the Heart Research Institute, Sydney, Australia.

Research Grants

2018-2020: Arterial dilating activity of an EPA metabolite, a novel endothelial-derived hyperpolarizing factor (EDHF), as an antihypertensive drug candidate. Israel Innovation Authority. In collaboration with Prof. Shaul Atar, Galilee Medical Center, Nahariya.

2016-2018: PON1 regulates blood pressure via EDHF. BSF- start-up grant. In collaboration with Prof. Gutterman David, Medical College of Wisconsin, Milwaukee, USA.

2015-2018: The regulation of 5,6-DHTL, a new endothelial-derived hyperpolarizing factor, by paraoxonase 1 in the modulation of vascular tone. ISF- Israel Science Foundation research fellowship.

2012-2015: A novel pathway for blood pressure regulation involving paraoxonase 1 and epoxygenase arachidonic acid metabolites. ISF - Israel Science Foundation research fellowship.

2012-2013: The Israeli Society for Research, Prevention, and Treatment of Atherosclerosis.

Scientific Publications

Fishing for lipid lactones using selective reaction and characteristic fragmentation pattern

Slutsky Smith EA, Khatib S, Szuchman-Sapir A.

Scientific Publications

Fishing for lipid lactones using selective reaction and characteristic fragmentation pattern

Paraoxonase 1 hydrolysis of EPA-derived lactone impairs endothelial-mediated vasodilation

Vasodilation and blood pressure-lowering effect mediated by 5,6-EEQ lactone in 5/6 nephrectomy hypertensive rats

The uptake mechanism and intracellular fate of Paraoxonase-1 in endothelial cells

Paraoxonase 1 in endothelial cells impairs vasodilation induced by arachidonic acid lactone metabolite.

5,6-?-DHTL, a stable metabolite of arachidonic acid, is a potential EDHF that mediates microvascular dilation.

In-vivo oxidized albumin? a pro-inflammatory agent in hypoalbuminemia.

Glabridin, an isoflavan from licorice root, up-regulates paraoxonase 2 expression under hyperglycemia and protects it from oxidation

Neutrophils recruited to the myocardium after acute experimental myocardial infarct generate hypochlorous acid that oxidizes cardiac myoglobin.

5,6-?-DHTL, a stable metabolite of arachidonic acid, is a potential substrate for paraoxonase 1

Human atherosclerotic plaque lipid extract impairs the antioxidant defense capacity of monocytes

Glabridin, a phytoestrogen from licorice root, up-regulates the intracellular antioxidant defense mechanism under glucose stress

Human atherosclerotic plaque lipid extract promotes expression of proinflammatory factors in human monocytes and macrophage-like cells

Oxysterols formation as a reflection of biochemical pathways: summary of in-vitro and in-vivo studies

Site ?specific hypochlorous acid-induced oxidation of recombinant human myoglobin affects specific amino acid residues and the rate of cytochrome b5-mediated heme reduction

Polymorphonuclear leukocyte phagocytic function increases in plasminogen knockout mice

Hypochlorous acid oxidizes methionine and tryptophan residues in myoglobin

Characterization of oxidative stress in blood from diabetic versus hypercholesterolemic patients, using a novel synthesized marker

Exogenous N-linoleoyl tyrosine marker as a tool for the characterization of cellular oxidative stress in macrophages

Exogenous N-linoleoyl tyrosine marker as a tool for the characterization of cellular oxidative stress in macrophages

Cholesterol, linoleic acid or/and tyrosine yield different spectra of products when oxidized alone or in a mixture: Studies in various oxidative systems