The principle of the DHPLC: Heteroduplex formation through hybridization after heating and cooling the PCR products. Separation of heteroduplexes from Homoduplexes is accomplished under partially denaturing conditions. Examples for the usage of the DHPLC:DHPLC detection of mutation in the Fanconi anemia gene, exon-4 in Ashkenazi population. DHPLC detection of new mutation at the BRCA2 gene, in Ashkenazi breast Cancer patients. The common mutation in this fragment is 6174 | 
