Dr. Ofri Levi

Dr. Ofri Levi
Principal Investigator
Research Group Leader
PhD
Phone
+972526264128
Principal Investigator

Regulation of Gene Expression in Fungal Resistance Mechanisms
Dr. Ofri Levi’s research addresses fungal resistance, a significant challenge with profound implications for both global agriculture and human health. The primary focus of the research is on elucidating the role of gene expression regulation in the development and persistence of antifungal resistance. In parallel, the lab is investigating the antifungal efficacy of plant-derived compounds using advanced genomic methodologies, particularly genome-wide CRISPR screens. By dissecting the genetic and molecular mechanisms underlying fungal resistance, Dr. Levi's work is dedicated to developing innovative and environmentally sustainable antifungal strategies.


Research Areas:

Antifungal Resistance Mechanisms: Uncovering the genetic factors and molecular pathways that enable fungi to develop resistance to both synthetic fungicides and natural antifungal agents.

  • CRISPRi-Based Genomic Screening: Utilizing CRISPRi technology to perform unbiased, high-throughput screening to identify novel molecular pathways involved in antifungal resistance.

  • Plant-Derived Compounds: Investigating the antifungal properties of plant-derived compounds and their potential as eco-friendly alternatives to synthetic fungicides.

     

CV

Academic Background
(2022 – 2024) Postdoctoral Researcher, Faculty of Biology, Technion, Israel 
Supervisor: Prof. Benjamin Horwitz
Research Focus: Genome-wide CRISPRi-based characterization of stress responses in fungi.

(2016 - 2022) Direct Ph.D. track in Biology, Technion, Israel 
Supervisor: Prof. Yoav Arava
Thesis: Roles of Aminoacyl tRNA synthetases in mRNA regulation.

Scientific Publications

Design, Synthesis, and Screening of an RNA Optimized Fluorinated Fragment Library

Kasper P. Lundquist, Emily S. Hudson, Marie L. Gram, Maëlle Pestalozzi, Raffaele B. Puglielli, Ofri Levi, Yoav S. Arava, Alina Vilensky, Timor Baasov, Charlotte H. Gotfredsen, and Mads H. Clausen
Under review
2024

Plant-fungal reciprocity of gene expression patterns in the maize-Cochliobolus heterostrophus interaction

Rina Zuchman, Ofri Levi, Adriana Rightmyer, Hee-Jin Park, Vivian Ng, Daniel Peterson, Roni Koren, Scott Baker, Benjamin A. Horwitz, B. Gillian Turgeon.
Under preparation
2024

Genome-Wide CRISPRi Screen Reveals Molecular Mechanisms of Ferulic Acid's Antifungal Action and Its Synergistic Potential with Fluconazole

Ofri Levi, Rina Zuchman, Roni Koren, Hazem Khamaisi and Benjamin A. Horwitz
Under preparation
2024

Cytoplasmic sequestering of a fungal stress-activated MAPK in response to a host plant phenolic acid

Rina Zuchman, Roni Koren, Tamar Ziv, Yael Lupu-Haber, Nitsan Dahan, Ofri Levi, and Benjamin A. Horwitz
Research Square Preprint
2024

ThrRS-mediated translation regulation of the RNA polymerase III subunit RPC10 occurs through an ASL-like structure and affects tRNA levels

Ofri Levi, Monalisha Mallik and Yoav Arava
Genes
2023

Comprehensive characterization of mRNAs associated with yeast cytosolic aminoacyl-tRNA synthetases

Shahar Garin, Ofri Levi, Megan E. Forrest, Anthony Antonellis, and Yoav S. Arava “” (2021)
RNA Biology
2023

Pseudouridine-mediated translation control by methionine aminoacyl tRNA synthetase

Ofri Levi and Yoav Arava
Nucleic Acids Research
2021

RNA modifications as a common denominator between tRNA and mRNA

Ofri Levi and Yoav Arava
Current Genetics
2021

Localization and RNA binding of mitochondrial aminoacyl tRNA synthetases

Shahar Garin, Ofri Levi, Bar Cohen, Adi Golani, and Yoav Arava
Genes
2020

Expanding the CRISPR/Cas9 toolbox for gene engineering in S. cerevisiae

Ofri Levi and Yoav Arava
Current Microbiology
2020

mRNA association by aminoacyl tRNA synthetase occurs at an anticodon mimic and autoregulates translation in response to tRNA levels

Ofri Levi and Yoav Arava
PLOS Biology
2019

RNA mimicry in post-transcriptional regulation by aminoacyl tRNA synthetases

Ofri Levi, Shahar Garin and Yoav Arava
WIREs RNA
2019