Dr. Ailie Marx

Dr. Ailie Marx
Research Group Leader
PhD

Lab Website

We are using computational tools, molecular dynamics, and experimental structural biology to explore how very local protein structure is formed. We are investigating how segments of the protein chain can adopt alternate local conformations, how cellular translation shape that process and the implications for protein function.

We are currently investigating the following specific research directions:
(a) We have developed an “amino-domino” model that uses amino acid pairs to model local sub-secondary structure and are now characterising the effect of adjacent amino acids on point mutations.
(b) We are looking at flexible protein sequences to develop models for very local protein structure that could be used to understand the behaviour of intrinsically disordered proteins.
(c) Using molecular dynamics we have identified intrinsically stable dual protein conformations. We are now using X-ray crystallography to follow the distribution of conformations under different expression and folding conditions. We are also using CryEM to image the nascent chains in the ribosome exit tunnel, probing for either uni or bimodal distributions of conformations.

Website ailiemarx.com

 

CV

Academic positions 
from 2023 Principal Investigator Migal – Galilee Research Institute
from 2024 Senior Lecturer Tel-Hai College
2020 – 2023 Researcher Bronstein Group, Department of Computer Science,Technion, Israel

2016 - 2020 Writing Consultant Research Authority, Technion, Israel.
2015 – 2016 Researcher Kaplan Group, Faculty of Biology, Technion, Israel

2011 – 2015 Postdoctoral Fellow Alian Group, Faculty of Biology, Technion, Israel
2002 Research Chemist Australian Therapeutic Goods Association Education 
2011 Ph.D. Faculty of Chemistry, Technion, Israel (Mentor: Noam Adir)
2008 M.A. Department of Natural Resources and Environmental Management, University of Haifa, Israel

2006 M.Sc. Faculty of Chemistry, Technion, Israel (Mentor: Noam Adir)
2003 B.Sc. Australian National University, Australia


Scientific Publications 
For a complete list of publications please see Dr. Marxs' Google Scholar page (https://scholar.google.co.il/citations?user=yTtz1QoAAAAJ&hl=en)

Awards and Honors 
2013 Best Presentation - Israeli Crystallography Association Meeting
2013 Most Outstanding Oral Presentation – Faculty of Biology Annual Retreat
2011 Klartag Prize for excellence in Biochemistry

Awarded once per year for outstanding graduate work in biochemistry

2009 Invited participant of the 59th Lindau Nobel Meeting

Reviewer as part of the alumni network
2008 Shulich Prize for Excellence in Teaching, Technion
I consistently obtain a top 10% score in teaching

Scientific Publications

Seeing Double: Molecular dynamics simulations reveal the stability of certain alternate protein conformations in crystal structures

Aviv A Rosenberg, Sanketh Vedula, Alex M Bronstein, Ailie Marx
bioRxiv
2024

A dataset of alternately located segments in protein crystal structures

Aviv A Rosenberg, Ailie Marx, Alexander M Bronstein
Scientific Data
2024

Water stabilizes an alternate turn conformation in horse heart myoglobin

Alex Bronstein, Ailie Marx
Scientific reports
2023

An amino-domino model described by a cross-peptide-bond Ramachandran plot defines amino acid pairs as local structural units

Aviv A Rosenberg, Nitsan Yehishalom, Ailie Marx, Alex M Bronstein
Proceedings of the National Academy of Sciences
2023

Humans as intuitive classifiers

Ido Erev, Ailie Marx
Frontiers in Psychology
2023

Machine learning approaches demonstrate that protein structures carry information about their genetic coding

Linor Ackerman-Schraier, Aviv A Rosenberg, Ailie Marx, Alex M Bronstein
Scientific Reports
2022

Defining amino acid pairs as structural units suggests mutation sensitivity to adjacent residues

Aviv A Rosenberg, Nitsan Yehishalom, Ailie Marx, Alex Bronstein
bioRxiv
2022

Codon-specific Ramachandran plots show amino acid backbone conformation depends on identity of the translated codon

Aviv A Rosenberg, Ailie Marx, Alex M Bronstein
Nature communications
2022

Recording Silence–Accurate Annotation of the Genetic Sequence Is Required to Better Understand How Synonymous Coding Affects Protein Structure and Disease

Aviv A Rosenberg, Alex M Bronstein, Ailie Marx
Single Nucleotide Polymorphisms: Human Variation and a Coming Revolution in Biology and Medicine
2022

Pausing kinetics dominates strand-displacement polymerization by reverse transcriptase

Omri Malik, Hadeel Khamis, Sergei Rudnizky, Ailie Marx, Ariel Kaplan
Nucleic acids research
2017

The preserved HTH-docking cleft of HIV-1 integrase is functionally critical

Meytal Galilee, Elena Britan-Rosich, Sarah L Griner, Serdar Uysal, Viola Baumgärtel, Don C Lamb, Anthony A Kossiakoff, Moshe Kotler, Robert M Stroud, Ailie Marx, Akram Alian
Structure
2016

Structure of FIV capsid C-terminal domain demonstrates lentiviral evasion of genetic fragility by coevolved substitutions

Aya Khwaja, Meytal Galilee, Ailie Marx, Akram Alian
Scientific Reports
2016

The first crystal structure of a dTTP-bound deoxycytidylate deaminase validates and details the allosteric-inhibitor binding site

Ailie Marx, Akram Alian
Journal of Biological Chemistry
2015

Rerouting Resistance: Escaping Restriction Using Alternative Cellular Pathways

Ailie Marx, Akram Alian
Trends in microbiology
2015

A novel open-barrel structure of octameric translin reveals a potential RNA entryway

Elad Eliahoo, Ailie Marx, Haim Manor, Akram Alian
Journal of Molecular Biology
2015

The road less traveled: HIV's use of alternative routes through cellular pathways

Ailie Marx, Akram Alian
Journal of virology
2015

Strand-displacement polymerization by Reverse Transcriptase: lessons from single molecule studies

O Malik, S Rudnizky, A Marx, A Kaplan
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
2015

Zinc enhancement of cytidine deaminase activity highlights a potential allosteric role of loop-3 in regulating APOBEC3 enzymes

Ailie Marx, Meytal Galilee, Akram Alian
Scientific reports
2015

Piecing together the phycobilisome

Ailie Marx, Liron David, Noam Adir
The Structural Basis of Biological Energy Generation
2014

Structural characteristics that stabilize or destabilize different assembly levels of phycocyanin by urea

Ailie Marx, Noam Adir
Photosynthesis research
2014

Allophycocyanin and phycocyanin crystal structures reveal facets of phycobilisome assembly

Ailie Marx, Noam Adir
Biochimica et Biophysica Acta (BBA)-Bioenergetics
2013

Crystallization of a giant photosynthetic antenna complex–the phycobilisome

Liron David, Xianglu Li, Ailie Marx, Robert Blankenship, Noam Adir
Acta Cryst
2011

Elucidation of Phycobilisome Functionalities Using High Resolution Structures of Phycobiliproteins

Ailie Marx
Technion-Israel Institute of Technology, Faculty of Chemistry
2011

High-Resolution Crystal Structures of Trimeric and Rod Phycocyanin

Liron David, Ailie Marx, Noam Adir
Journal of molecular biology
2011

Allophycocyanin Trimer Stability and Functionality Are Primarily Due to Polar Enhanced Hydrophobicity of the Phycocyanobilin Binding Pocket

Ailie McGregor, Merav Klartag, Liron David, Noam Adir
Academic Press
2008